Marlene is a female otter who was transferred to the Central Park Zoo from an aquarium in California. She is close friends with Skipper and sometimes tags along on his missions, but she is often a neutral character who does not take sides between the penguins and the lemurs. By being a levelheaded, feminine voice of reason, she is a counterpoint to Skipper's masculine, covert-ops character. In Manny, Sid, and Diego's Adventures of We're Back! A Dinosaur's Story, it is revealed that Marlene turned wild after eating Professor Screweyes’ Brain Drain along with Rex, Dweeb, Woog, Elsa, Diego, Shira, and Tigress, but they eventually gone back to normal. She can be a niece to team like Ellie. Marlene is voiced by Nicole Sullivan.
- She will join the Sub-zero Group in Manny, Sid, and Diego's Adventures of Madagascar.