Charlie Barkin and Itchy Itchiford are the two dogs who they first met the Sub-zero Squad and two of the main protagonists in Manny, Sid, and Diego's Adventures of All Dogs Go to Heaven.
Charlie Barkin - (one of the main protagonists) a German Shepherd who became an angel, along with his best friend, Itchy Itchford. He and Itchy became friends with the Sub-Zero Adventure Group. Charlie is voiced by Burt Reynolds in the first film, spoken by Charlie Sheen and sung by Jesse Corti in the second film, and both spoken and sung by Steven Weber in the rest of the franchise.
Itchy Itchiford - (one of the main protagonists) He's Charlie Barkin's assistant and close friend. He has a problem keeping his fleas under control, and is often the only voice of reason in Charlie's schemes. He is another old friend of the Sub-zero Group and his friends in Manny, Sid, and Diego's Adventures of All Dogs Go to Heaven. Itchy is voiced by the late Dom DeLuise.
- At the end of Manny, Sid, and Diego's Adventures of All Dogs Go to Heaven 2, Charlie and Itchy will join their team.
- Charlie and Itchy will make their guest appearances in Danny Phantom and Sabrina the Teenage Witch in Friends Forever, Kim Possible's Adventures of The Super Mario Bros. Movie, and more.